Rapid screening for Chlamydia trachomatis infection by detecting α-mannosidase activity in urogenital tract specimens
نویسندگان
چکیده
BACKGROUND Chlamydia trachomatis may cause multiple different urogenital tract disorders, but current non-culture assays for rapid screening of C. trachomatis typically use immunochromatography-based methods. We established another new rapid non-culture method for detection of C. trachomatis based on the measurement of α-mannosidase enzymatic activity in urogenital tract specimens. METHOD To evaluate the performance of this method, α-mannosidase activities of C. trachomatis serotype D strain 、 and 29 standard strains related to clinical urogenital pathogens were investigated. Furthermore, 553 urogenital tract specimens were used for clinical assays via cell culture method and ligase chain reaction method (LCR), adopting an expanded gold standard. RESULTS Only C. trachomatis was positive for α-mannosidase activity among different types of microbes tested in the research. When prostate fluid specimens, which have some interfering activity, were excluded, the sensitivity and specificity of the enzymatic method were 91.8% (78/85) and 98.3% (409/416), respectively. There were no significant differences (P > 0.05). CONCLUSIONS These results showed that α-mannosidase activity could be utilised as a screening marker of C. trachomatis infection.
منابع مشابه
Molecular Evidence of Chlamydia trachomatis Infection and its Relation to Miscarriage
Objective Chlamydia trachomatis (CT) infection is the most common sexually transmitted disease in the world that can persist and also ascend in the genital tract. This intracellular and silent infection is related to some adverse pregnancy outcomes, such as miscarriage. The aims of this study were to explore the best CT screening tests using blood and vaginal samples and to investigate the corr...
متن کاملP-204: Relative Frequency of Chlamydia Trachomatis Infection in Infertile Women
Background: The majority of Chlamydia trachomatis infections in women are asymptomatic, but they may give rise to tubal infertility. Screening programmes aim at reducing morbidity in individual by early detect and treatment, and at decreasing the overall prevalence of infection in the population. Materials and Methods: A total of 135 women presenting with a history of infertility(n=113(83.7%) p...
متن کاملupper genital tract infection : A review
Upper genital tract infection causes inflammation in multiple organs such as endometrium, fallopian tubes, ovaries, and pelvic peritoneum. This type of disease is caused due to infection with cervical pathogens, such as Neisseria gonorrhea, Chlamydia trachomatis, Mycoplasma genitalium, bacterial vaginosis pathogens, and respiratory pathogens. The pathogens which involve the respiratory system a...
متن کاملScreening of Chlamydia trachomatis urogenital infections among the male and female population of the Republic of Macedonia.
BACKGROUND Noninvasive urine screening for Chlamydia trachomatis infections offers a valuable public health tool, that could be of vast importance in Chlamydia control programs. OBJECTIVE The goal was to determine the prevalence of C. trachomatis infections among a sexually active population, to define the epidemiological factors associated with it, and to develop potential selective screenin...
متن کاملThe Prevalence of Chlamydia trachomatis Infection in Women Attending Health Clinics in Shiraz, Islamic Republic of Iran
Background Chlamydia trachomatis (CT) is a common cause of sexually transmitted infections (STI) and a prevalent microorganism found in the vaginal discharge of sexually active women. The infection usually has no symptoms; although it may cause chronic complications such as pelvic inflammatory disease (PID), ectopic pregnancy, chronic pelvic pain, tubal infertility and cervical cancer. This stu...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
دوره 13 شماره
صفحات -
تاریخ انتشار 2013